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Composite

Part:BBa_K1139110:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-09)


Pcon-LasR-Plux/tet-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 927
    Illegal BsaI.rc site found at 1675


Design Notes

sequence confirmed

Source

Composite of BBa_K553003 and BBa_K934025.

References

1. Gray KM, Passador L (1994) Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa. Journal of bacteriology 176(10): 3076–3080.
2. Hideki Kobayashi (2004) Programmable cells: Interfacing natural and engineered gene networks. vol. 101 no. 22 8414–8419